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MICROBIOLOGY



Pathology Lab in Nepal Img-1
MICROBIOLOGY

The Crystal Diagnostic Pvt. Ltd laboratory is a full service laboratory offering comprehensive testing for the diagnosis of infectious diseases. Infectious disease may be acute or chronic and may be caused by a variety of microorganisms that may include bacteria, viruses and parasites. Our staff of experienced clinical microbiologists has been trained in the most up to date diagnostic methods for the detection and testing of microbial pathogens in the areas of:

  • bacteriology
  • antimicrobial susceptibility
  • molecular methods
  • mycobacteriology
  • rapid serological identification
  • parasitology

Diagnosis of immunological and allergic disorders using serological tests e.g. Viral serology for Dengue virus and Antigen detection, Human Immunodeficiency virus, Hepatitis viruses A, B, C, E, Tuberculosis and TORCH panel.

Culture based diagnosis of aerobic microorganisms by convention and automated methods (automated blood culture system BD BACTEC FX40).

  • Bacteriology is the branch and specialty of biology that studies the morphology, ecology, genetics and biochemistry of bacteria as well as many other aspects associated with them. The identification, classification and characterization of bacterial organisms is part of this subdivision of microbiology.

In order to determine which specific antibiotics a specific bacterium is susceptible to, antimicrobial susceptibility tests are used. This test most frequently complements a gram stain and culture, whose findings are obtained much sooner. Tests of antimicrobial susceptibility will guide the doctor in the choice of drugs and dosage for infections that are difficult to treat.

  • Molecular diagnostics are rapidly becoming a traditional laboratory technique for a large number of diseases. Several DNA-based molecular methods are used in clinical practice. In addition to the diagnosis of a specific disease in particular patient. In certain cases, the diagnosis is based on the identification of mutations in the genes involved, although variations in the number of DNA copies can be identified in some cases, leading to changes in gene expression.
  • Mycobacteriology is the study and assessment of clinical aspects of mycobacteria. This deals with all the clinical research and methods conducted for the understanding of mycobacterium species. Mycobacteria are the causative agent of several fatal diseases such as leprosy and tuberculosis.
  • Serologic tests are particularly useful for species that are difficult to cultivate by routine laboratory methods. Serological tests are used in microbiology to assess if an organism has antibodies to a particular pathogen. The presence of antibodies in the blood of an individual against a pathogen suggests they have been exposed to that pathogen. Shortly after a person is exposed to the pathogen, IgM is produced in large amounts. Upon first exposure, IgG is also produced, but not as quickly as IgM. For an extended period of time, IgG remains in circulation.

 

Routinely Performed Test in Bacteriology

S.N TEST SAMPLE TYPE
1 AFB EXAMINATION ( 3 DAYS) SPUTUM
2 AFB STAIN (PUS) PUS
3 ASCITIC FLUID CULTURE AND SENSITIVITY ASCITIC FLUID
4 BLOOD CULTURE AND SENSITIVITY WHOLE BLOOD IN BHI
5 BODY FLYIDS CULTURE AND SENSITIVITY BODY FLUIDS
6 CSF CULTURE AND SENSITIVITY CSF
7 GRAM STAIN (SPUTUM) SPUTUM
8 GRAM STAIN (URETHRAL DISCHARGE ) URETHRAL DISCHARGE
9 HANGING DROP PREPARATION (STOOL) STOOL
10 HIGH VAGINAL SWAB CULTURE AND SENSITIVITY VAGINAL SWAB
11 PLEURAL FLUID CULTURE AND SENSITIVITY PLEURAL FLUID
12 SEMEN CULTURE AND SENSITIVITY SEMEN
13 PUS CULTURE AND SENSITIVITY PUS
14 SPUTUM AFB FOR 1 DAY SPUTUM
15 SPUTUM AFB FOR 2 DAY SPUTUM
16 SPUTUM AFB FOR 3 DAY SPUTUM
17 SPUTUM FOR FUNGUS SPUTUM
18 STOOL CULTURE AND SENSITIVITY STOOL
19 URINE CULTURE AND SENSITIVITY URINE

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